Journal: Amino Acids

Article Title: Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation

doi: 10.1007/s00726-014-1857-1

Figure Lengend Snippet: Effect of arginine deprivation on glioblastoma cells. a Cell growth of U251 ( upper panel ) and U87 ( lower panel ) cells cultivated in control, -Arg, -Lys and conditions. b U251 cell viability assessed under deprivation and re-supplementation conditions, as indicated. Upper and lower panels , arginine and lysine re-supplementation, respectively. 100 %, the number of the viable cells at time 0. Data in a and b are means ± SD; *** and *Statistical relevance p < 0.001 and p < 0.05, respectively

Article Snippet: Human glioblastoma U251 MG (U251) and U87 MG (U87) cell lines were purchased from CLS Cell Lines Service (Germany) and monitored for correct genetic profile via microsatellite analyses according to a protocol described earlier (Peickert et al. ).

Techniques:

Journal: Amino Acids

Article Title: Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation

doi: 10.1007/s00726-014-1857-1

Figure Lengend Snippet: Arginine deprivation affects morphology of glioblastoma but not glia cells. a , b , d Rat glia, U251 and U87 cells stained with Alexa 488-phalloidin, respectively. c Micrographs of U251 cells attained with scanning electron microscope. Insets in a , b and d ~2–3× magnification of the marked areas . Bars , in a , b and d 50 μm, and in c 10 μm. e U251 cells stained with Alexa 488-phalloidin before and after re-supplementation with Arg or Lys up to 0.4 and 0.8 mM concentration, respectively. Arrows point to lamellipodia, arrowheads point to elongated cells

Article Snippet: Human glioblastoma U251 MG (U251) and U87 MG (U87) cell lines were purchased from CLS Cell Lines Service (Germany) and monitored for correct genetic profile via microsatellite analyses according to a protocol described earlier (Peickert et al. ).

Techniques: Staining, Microscopy, Concentration Assay

Journal: Amino Acids

Article Title: Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation

doi: 10.1007/s00726-014-1857-1

Figure Lengend Snippet: Arginine deprivation impairs cell motility. a , b Migration tracks of U251 and U87 cells, respectively. Upper panels in a and b tracks of 10 randomly chosen cells; center panels images of migrating cells, and lower panels values of migration rate and mean distance based on tracks shown in upper panels . Values are means ± SD. ***Statistical relevance p < 0.001

Article Snippet: Human glioblastoma U251 MG (U251) and U87 MG (U87) cell lines were purchased from CLS Cell Lines Service (Germany) and monitored for correct genetic profile via microsatellite analyses according to a protocol described earlier (Peickert et al. ).

Techniques: Migration

Journal: Amino Acids

Article Title: Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation

doi: 10.1007/s00726-014-1857-1

Figure Lengend Snippet: Arginine deprivation impairs cell migration and invasiveness. Transwell filters not covered ( a ), and covered with Matrigel ( b , c) were used for analyses. Upper and lower panels in a and b images of U251 and U87 stained cells, respectively, taken on the filter trans side. c Images of LN-229 cells, analyzed as in b . Analyses were performed for three independent experiments run in duplicates. d Images of GFP-expressing U251 cells found within the E13 organotypic brain slice. The images represent the confocal 12.3-μm z -section of the planar center of brain slices. Right panel the quantification of GFP-expressing U251 cells within the confocal center of the slice per view area. The quantitative data in a – d are presented as % of control. Values are means ± SD. ***Statistical relevance p < 0.001

Article Snippet: Human glioblastoma U251 MG (U251) and U87 MG (U87) cell lines were purchased from CLS Cell Lines Service (Germany) and monitored for correct genetic profile via microsatellite analyses according to a protocol described earlier (Peickert et al. ).

Techniques: Migration, Staining, Expressing, Slice Preparation

Journal: Oncology Letters

Article Title: miR-9-3p inhibits glioma cell proliferation and apoptosis by directly targeting FOXG1

doi: 10.3892/ol.2020.11725

Figure Lengend Snippet: Effects of miR-9-3p overexpression on the proliferation of U87 cells. U87 cells were transfected with miR-9-3p mimic and NC mimic (50 pmol/ml) for 24 h. (A and C) RT-qPCR was applied to quantify the expression level of miR-9-3p in U87 MG and TG-905 cells. (B and D) The cell viability of U87 cells was determined by a CCK-8 assay in U87 MG and TG-905 cells. ##P<0.01 vs. NC mimic group. miR-9-3p, microRNA-9-3p; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; NC, negative control; control, without any treatment.

Article Snippet: Cell culture and transfection The human glioblastoma U87 MG (CL-0238) and TG-905 (CL-0309) cell lines were purchased from Procell Life Science & Technology Co., Ltd. (the origin of glioblastoma U87 MG cell is unknown and the cell line is preserved at the ATCC).

Techniques: Over Expression, Transfection, Quantitative RT-PCR, Expressing, CCK-8 Assay, Real-time Polymerase Chain Reaction, Negative Control

Journal: Oncology Letters

Article Title: miR-9-3p inhibits glioma cell proliferation and apoptosis by directly targeting FOXG1

doi: 10.3892/ol.2020.11725

Figure Lengend Snippet: Effects of miR-9-3p overexpression on the apoptosis of U87 cells. U87 cells were transfected with miR-9-3p mimic and NC mimic (50 pmol/ml) for 24 h. (A) Annexin-V/PI double-staining assay was applied to detect the cell apoptotic rate in U87 MG cells. (B) Annexin-V/PI double-staining assay was applied to detect the cell apoptotic rate in TG-905 cells. ##P<0.01 vs. NC mimic group. miR-9-3p, microRNA-9-3p; NC, negative control; PI, propidium iodide; control, without any treatment.

Article Snippet: Cell culture and transfection The human glioblastoma U87 MG (CL-0238) and TG-905 (CL-0309) cell lines were purchased from Procell Life Science & Technology Co., Ltd. (the origin of glioblastoma U87 MG cell is unknown and the cell line is preserved at the ATCC).

Techniques: Over Expression, Transfection, Double Staining, Negative Control

Journal: Oncology Letters

Article Title: miR-9-3p inhibits glioma cell proliferation and apoptosis by directly targeting FOXG1

doi: 10.3892/ol.2020.11725

Figure Lengend Snippet: FOXG1 is a direct target of miR-9-3p in glioma cells. (A) Bioinformatics analysis of the predicted interactions of miR-9-3p and its binding sites within the 3′-UTR of FOXG1. (B) Relative luciferase activities of FOXG1-wt, and FOXG1-mut were identified using a Dual-Luciferase Reporter assay kit following transfection of U87 MG with miR-9-3p mimic and NC mimic (50 pmol/ml) for 24 h. (C) The expression level of FOXG1 mRNA was examined by RT-qPCR following transfection of U87 MG with miR-9-3p mimic and NC mimic (50 pmol/ml) for 24 h. (D) The expression level of FOXG1 protein was examined by western blot assay following transfection of U87 MG with miR-9-3p mimic and NC mimic (50 pmol/ml) for 48 h. The relative intensity of FOXG1 protein is presented as a bar graph. (E) Relative luciferase activities of FOXG1-wt, and FOXG1-mut were identified using a Dual-Luciferase Reporter assay kit following transfection of TG-905 cells with miR-9-3p mimic and NC mimic (50 pmol/ml) for 24 h. (F) The expression level of FOXG1 mRNA was examined by RT-qPCR following transfection of TG-905 cells with miR-9-3p mimic and NC mimic (50 pmol/ml) for 24 h. (G) The expression level of FOXG1 protein was examined by western blot assay following transfection of TG-905 cells with miR-9-3p mimic and NC mimic (50 pmol/ml) for 48 h. The relative intensity of FOXG1 protein is presented as a bar graph. *P<0.05, **P<0.01 vs. NC mimic group. FOXG1, forkhead box G1; miR-9-3p, microRNA-9-3p; 3′-UTR, 3′-untranslated region; wt, wild-type; mut, mutant; NC, negative control.

Article Snippet: Cell culture and transfection The human glioblastoma U87 MG (CL-0238) and TG-905 (CL-0309) cell lines were purchased from Procell Life Science & Technology Co., Ltd. (the origin of glioblastoma U87 MG cell is unknown and the cell line is preserved at the ATCC).

Techniques: Binding Assay, Luciferase, Reporter Assay, Transfection, Expressing, Quantitative RT-PCR, Western Blot, Mutagenesis, Negative Control

Journal: Oncology Letters

Article Title: miR-9-3p inhibits glioma cell proliferation and apoptosis by directly targeting FOXG1

doi: 10.3892/ol.2020.11725

Figure Lengend Snippet: miR-9-3p regulates cell proliferation by inhibiting FOXG1. (A and C) Western blotting was used to detect the protein expression of FOXG1 following transfection of U87 MG and TG-905 cells with FOXG1 siRNA (40 pmol/ml) for 48 h. (B and D) Relative cell viability was determined by a Cell Counting Kit-8 assay following transfection of U87 MG and TG-905 cells with FOXG1 (40 pmol/ml) for 24 h. **P<0.01 vs. control group; ##P<0.01 vs. miR-9-3p mimic group; &&P<0.01 vs. FOXG1-siRNA group. FOXG1, forkhead box G1; miR-9-3p, microRNA-9-3p; siRNA, small interfering RNA; control, without any treatment.

Article Snippet: Cell culture and transfection The human glioblastoma U87 MG (CL-0238) and TG-905 (CL-0309) cell lines were purchased from Procell Life Science & Technology Co., Ltd. (the origin of glioblastoma U87 MG cell is unknown and the cell line is preserved at the ATCC).

Techniques: Western Blot, Expressing, Transfection, Cell Counting, Small Interfering RNA

Journal: Oncology Letters

Article Title: miR-9-3p inhibits glioma cell proliferation and apoptosis by directly targeting FOXG1

doi: 10.3892/ol.2020.11725

Figure Lengend Snippet: miR-9-3p regulates cell apoptosis by inhibiting FOXG1. (A) Annexin-V/PI double-staining assay was performed to detect the cell apoptotic rate of U87 MG cells following transfection with FOXG1 siRNA (40 pmol/ml) for 24 h. (B) Annexin-V/PI double-staining assay was applied to detect the cell apoptotic rate of TG-905 cells following transfection with FOXG1 siRNA (40 pmol/ml) for 24 h. **P<0.01 vs. control group; ##P<0.01 vs miR-9-3p mimic group; &&P<0.01 vs. FOXG1-siRNA group. miR-9-3p, microRNA-9-3p; FOXG1, forkhead box G1; PI, propidium iodide; siRNA, small interfering RNA; control, without any treatment.

Article Snippet: Cell culture and transfection The human glioblastoma U87 MG (CL-0238) and TG-905 (CL-0309) cell lines were purchased from Procell Life Science & Technology Co., Ltd. (the origin of glioblastoma U87 MG cell is unknown and the cell line is preserved at the ATCC).

Techniques: Double Staining, Transfection, Small Interfering RNA